防哮饮早期干预对哮喘小鼠血清白细胞介素10水平影响的实验研究

中国中西医结合儿科学 ›› 2011, Vol. 3 ›› Issue (3): 203-205.

防哮饮早期干预对哮喘小鼠血清白细胞介素10水平影响的实验研究

宾博平，刘孝忠，李亚俊，黄小琪，梁丽英

530023 南宁，广西中医学院第一附属医院哮喘及过敏性疾病研究所（宾博平）；312500 浙江绍兴，浙江省新昌县人民医院儿科（刘孝忠）；530001 南宁，广西中医学院（李亚俊）；530023 南宁，广西中医学院第一附属医院综合实验室（黄小琪，梁丽英）

出版日期:2011-06-25 上线日期:2019-05-27
作者简介:宾博平（1962-），男，副教授、副主任医师。研究方向：儿童哮喘及过敏性疾病，Email：bbp168@163.com。
基金资助:
广西科学基金项目(桂科基0448062)

Experimental study on the effect of early interference of Fangxiaoyin Decoction on serum Interleukin10 level in asthmatic mice

BIN Boping,LIU Xiaozhong,LI Yajun,et al.

Asthmatic and Allergic Disease Institute of the First Affiliated Hospital of Guangxi College of TCM,Nanning 530023,China

Published:2011-06-25 Online:2019-05-27

摘要/Abstract

摘要： 目的观察中药“防哮饮”早期干预对哮喘小鼠血清白细胞介素10（IL10）水平以及支气管肺泡灌洗液（BALF）中嗜酸性粒细胞百分比（EOS%）的影响。
方法将60只健康昆明小鼠随机分为6组：正常对照组、哮喘模型组、“防哮饮”小剂量组、“防哮饮”中剂量组、“防哮饮”大剂量组、布地奈德组各10只，用卵蛋白致敏激发造模。中药“防哮饮”组从致敏第1天起，将中药“防哮饮”按小剂量（含生药0.5 g/mL）、中剂量（含生药1.5 g/mL）、大剂量（含生药2.5 g/mL）灌胃给药，每日1次，每次0.4 mL，共27 d。在最后1次激发后24 h(第28 d)处死小鼠，采用双抗夹心酶联免疫吸附试验检测小鼠血清IL10的含量，并镜检BALF中EOS%。
结果哮喘模型组小鼠BALF中EOS［（2.9±1.4）%］较对照组［（1.3±0.9）%］明显升高（P＜0.01）；血清IL10的含量［（7.6±3.0）ng/mL］较对照组［（44.9±33.0）ng/mL］降低（P＜0.05）。“防哮饮”小、中、大剂量组均能显著提高哮喘小鼠血清IL10水平，与哮喘模型组比较差异有统计学意义（P＜0.01），与布地奈德组比较差异无统计学意义（P＞0.05）。“防哮饮”大、中、小剂量组和布地奈德组均能明显降低BALF中EOS%（P＜0.01），各组间差异无统计学意义（P＞0.05）。结论中药“防哮饮”及布地奈德早期干预治疗均能够明显改善哮喘小鼠病理状态，上调哮喘小鼠血清IL10表达和降低BALF中EOS%水平可能是其作用机制。

关键词: 防哮饮；哮喘；白细胞介素10；小鼠, 近交系；动物, 实验

Abstract: To investigate the effects of Fangxiaoyin Decoction on the serum concentration of Interleukin10(IL10) and eosinophil of bronchoalveolar lavage fluid in asthmatic mice after early interference treatment.〖WTHZ〗Methods〖WTBZ〗Sixty healthy Kunming mice were randomly divided into six groups: Group A:normal control group,Group B:asthmatic model group,Group C:lowdose Fangxiaoyin decoction treatment group,Group D:mediumdose Fangxiaoyin decoction treatment group,Group E:highdose Fangxiaoyin decoction treatment group,Group F:Budesonide(BUD)treatment group.The asthmatic mice model was stimulated with ovalumin.Fangxiaoyin decoction treatment groups were given from the first day sensitization by lowdose (containing crude drug 0.5 g/mL),medium dose (containing crude drug 1.5 g/mL),highdose (containing crude drug 2.5 g/mL),once a day,each 0.4 mL,for a total of 27 days.At the 24 h after the final stimulation (28 days),the mice were executed.Detect IL10 by ELISA.And then,detect the percentage of EOS of BALF by microscope.〖WTHZ〗Results〖WTBZ〗The percentage of EOS of BALF in group B was significantly higher than group A(P<0.01).The concentration of IL10 in group B was significantly lower than group A(P<0.01).Group C,D and E the concentrations of IL10 were significantly increased.There was significant difference between groups C,D,E and B(P<0.01) on the concentrations of IL10,and there was no significant difference between groupC,D,E and F(P>0.05).In group C,D,E and F EOS% of BALFwas decreased(P<0.01),there being no significant difference among the groups(P>0.05).〖WTHZ〗Conclusion〖WTBZ〗Pathological state of asthmatic mice can be greatly improved after early interference treatment by Fangxiaoyin Decoction and BUD.The mechanism may be to increase IL10 and decrease EOS% of BALF.

Key words: Fangxiaoyin Decoction;Asthma;Interleukin10;Mice, inbred strain;Animal,experiment

宾博平，刘孝忠，李亚俊，黄小琪，梁丽英. 防哮饮早期干预对哮喘小鼠血清白细胞介素10水平影响的实验研究[J]. 中国中西医结合儿科学, 2011, 3(3): 203-205.

BIN Boping,LIU Xiaozhong,LI Yajun,et al.. Experimental study on the effect of early interference of Fangxiaoyin Decoction on serum Interleukin10 level in asthmatic mice[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2011, 3(3): 203-205.

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