吲哚胺2，3-双加氧酶在哮喘小鼠肺组织的表达及与γ干扰素的关系

中国中西医结合儿科学 ›› 2021, Vol. 13 ›› Issue (2): 103-.

吲哚胺2，3-双加氧酶在哮喘小鼠肺组织的表达及与γ干扰素的关系

许春娜，李晓，汤昱，张磊，王秀芳

450053 郑州，郑州大学附属儿童医院呼吸科

出版日期:2021-04-25 发布日期:2021-05-18
通讯作者: 许春娜，E-mail：454365930qq.com
作者简介:许春娜（1989-），女，医学硕士，医师。研究方向：小儿呼吸与哮喘疾病的诊治
基金资助:
河南省医学科技攻关计划（2018020621）

IDO expression in lung tissue and its relationship with IFN-γ in asthmatic mice

XU Chunna，LI Xiao，TANG Yu，ZHANG Lei，WANG Xiufang

Department of Pediatrics， Affiliated Children's Hospital of Zhengzhou University， Zhengzhou 450053，China

Online:2021-04-25 Published:2021-05-18

摘要/Abstract

摘要： 目的:检测小鼠肺组织中吲哚胺2，3-双加氧酶(IDO)的表达和血清及支气管肺泡灌洗液（BALF）中γ干扰素(IFN-γ)的水平，探讨IDO在支气管哮喘发病机制中的作用与IFN-γ的关系。
方法:选择SPF级BALB/C小鼠21只，随机分成对照组、干预组、哮喘组各7只。尘螨提取液致敏、激发建立小鼠哮喘模型，苏木精-伊红染色观察气道炎症细胞改变，BALF行细胞学分析，ELISA法检测BALF中IFN-γ水平，免疫组织化学、Western Blot检测肺组织IDO蛋白的表达。
结果:哮喘组：炎症细胞浸润、气道的平滑肌细胞增生、管腔狭窄均明显；干预组：少量炎症细胞侵润，有气道平滑肌细胞的增生，管腔稍狭窄；对照组：无明显变化。哮喘组小鼠BALF中细胞总数、嗜酸性粒细胞数、中性粒细胞数高于干预组及对照组，差异均有统计学意义（P<0.05）。哮喘组血清及BALF中IFNγ水平低于干预组及对照组，差异均有统计学意义（P<0.05）。哮喘组肺组织IDO灰度值低于对照组及干预组，差异均有统计学意义（P<0.05）。IFN-γ水平在血清中与BALF中呈正相关（P<0.01）；肺组织内IDO蛋白的表达与IFN-γ水平在血清中及BALF中均呈正相关（P<0.01）。
结论:小鼠肺组织中IDO蛋白表达量降低，导致体内免疫耐受缺陷和（或）减弱，促使支气管哮喘的发生发展。

关键词: 哮喘, 吲哚胺2, 3-双加氧酶, IFN-γ

Abstract: Objective:To detect the expression of indoleamine 2,3-dioxygenase (IDO)in lung tissue and the level of interferon γ(IFN-γ) in serum and bronchoalveolar lavage fluid(BALF), and to explore the role of IDO in the pathogenesis of bronchial asthma and its relationship with IFN-γ.
Methods:Select 21 SPF BALB/C mice. They were randomly divided into control group, intervention group and asthma group, with 7 mice in each group. A mouse model of asthma was established by sensitization and stimulation of dust mites extract. The changes of airway inflammatory cells were observed by hematoxylineosin staining; BALF was given cytological analysis; ELISA was used to detect IFN-γ levels in BALF; immunohistochemistry and Western Blot were used to detect IDO protein expression in lung tissue.
Results:Asthma group: inflammatory cell infiltration, airway smooth muscle cell proliferation and lumen stenosis were obvious; intervention group: there were a small number of inflammatory cells infiltrating, there was airway smooth muscle cell proliferation, and lumen slightly narrowed; control group: there were no significant changes. The total number of cells, eosinophils and neutrophils in the BALF of asthma group were higher than those in the intervention group and the control group (P<0.05). The serum and BALF IFN-γ levels in asthma group were lower than those in intervention group and control group (P<0.05). The IDO gray value of lung tissue in asthma group was lower than that in control group and intervention group (P<0.05). IFN-γ level in serum was positively correlated with that in BALF(P<0.01), and expression of IDO protein in lung tissue was positively correlated with IFN-γ level in serum and BALF (P<0.01).
Conclusion:There is a decrease in IDO protein expression in lung tissue of mice, which leads to deficiency and/or decrease of immune tolerance in vivo and promotes the development of bronchial asthma.

Key words: Asthma, Indoleamine 2, 3-dioxygenase, IFN-γ

许春娜，李晓，汤昱，张磊，王秀芳. 吲哚胺2，3-双加氧酶在哮喘小鼠肺组织的表达及与γ干扰素的关系[J]. 中国中西医结合儿科学, 2021, 13(2): 103-.

XU Chunna，LI Xiao，TANG Yu，ZHANG Lei，WANG Xiufang. IDO expression in lung tissue and its relationship with IFN-γ in asthmatic mice[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2021, 13(2): 103-.

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