ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2021, Vol. 13 ›› Issue (3): 231-233.

• 临床研究 • 上一篇    下一篇

EV71型IgG、IgM和IgA抗体在手足口病诊断中的意义

目的: 探究EV71型IgG、IgM和IgA抗体在手足口病诊断中的应用价值。
方法: 以2019年3月至2020年3月在我院收治的EV71感染的手足口病患儿116例，通过实时荧光定量聚合酶链式反应法对患儿感染的病毒类型进行检测，使用免疫层析法检测患儿血清中的EV71型IgG、IgM和IgA抗体。
结果: EV71型IgG、IgM和IgA抗体的阳性检出率分别为62.07%（72/116）、68.10%（79/116）和60.34%（70/116），与EV71核酸检测的阳性检出率72.41%（84/116）比较，差异无统计学意义（P>0.05）。轻症患儿的EV71型IgM和IgG抗体阳性检出率低于重症患儿，差异有统计学意义（P<0.01）。
结论: EV71型IgG、IgM和IgA抗体检测对手足口病显示出较好的诊断能力，重症EV71型IgM和IgG抗体阳性率高于普通患儿，提示对疾病的早期诊断和对症治疗有临床应用价值。   

  1. 518117 广东 深圳，深圳市龙岗区坪地预防保健所质量管理科检验室
  • 出版日期:2021-06-25 上线日期:2021-12-15
  • 通讯作者: 赖芳，E-mail：zhaolikk9@163.com
  • 作者简介:赖芳（1974-），男，主管技师。研究方向：微生物检验及肠道致病菌的鉴定和分离
  • 基金资助:
    深圳市科技计划项目（201103246）

Significance of EV71 IgG, IgM and IgA antibodies in the diagnosis of hand-foot-mouth disease

Objective: To explore the application value of EV71 IgG, IgM and IgA antibodies in the diagnosis of hand-foot-mouth disease.#br# Methods :Totally 116 cases of children with hand-foot-mouth disease(HFMD) infected with EV71 who were treated in our hospital from March 2019 to March 2020 were selected, and the type of virus was detected by real-time fluorescent quantitative PCR, and the EV71 IgG, IgM and IgA antibodies were detected by immunochromatography.#br# Results: The positive rates of EV71 IgG, IgM and IgA antibodies were 62.07%(72/116), 68.10%(79/116) and 60.34%(70/116), respectively,and the positive rate of EV71 nucleic acid test was 72.41%(84/116), the difference being not statistically significant(P>0.05).The positive rate of EV71 IgM and IgG antibody in mild children was lower than that in severe children,and the difference was statistically significant(P<0.01).#br# Conclusion: The diagnostic ability of the detection of EV71 IgG, IgM and IgA antibodies in hand-foot-mouth disease is good; the positive rate of IgM and IgG antibody of severe EV71 is higher than that of common children, which suggests that it has clinical application value for early diagnosis and symptomatic treatment of the disease.   

  1. Department of Laboratory of Quality Management,Pingdi Preventive Health Care Center of Longgang District, Shenzhen 518117,China
  • Published:2021-06-25 Online:2021-12-15

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摘要： 目的: 探究EV71型IgG、IgM和IgA抗体在手足口病诊断中的应用价值。
方法: 以2019年3月至2020年3月在我院收治的EV71感染的手足口病患儿116例，通过实时荧光定量聚合酶链式反应法对患儿感染的病毒类型进行检测，使用免疫层析法检测患儿血清中的EV71型IgG、IgM和IgA抗体。
结果: EV71型IgG、IgM和IgA抗体的阳性检出率分别为62.07%（72/116）、68.10%（79/116）和60.34%（70/116），与EV71核酸检测的阳性检出率72.41%（84/116）比较，差异无统计学意义（P>0.05）。轻症患儿的EV71型IgM和IgG抗体阳性检出率低于重症患儿，差异有统计学意义（P<0.01）。
结论: EV71型IgG、IgM和IgA抗体检测对手足口病显示出较好的诊断能力，重症EV71型IgM和IgG抗体阳性率高于普通患儿，提示对疾病的早期诊断和对症治疗有临床应用价值。

关键词: 手足口病, EV71, IgG抗体, IgM抗体, IgA抗体, 儿童

Abstract: Objective: To explore the application value of EV71 IgG, IgM and IgA antibodies in the diagnosis of hand-foot-mouth disease.
Methods :Totally 116 cases of children with hand-foot-mouth disease(HFMD) infected with EV71 who were treated in our hospital from March 2019 to March 2020 were selected, and the type of virus was detected by real-time fluorescent quantitative PCR, and the EV71 IgG, IgM and IgA antibodies were detected by immunochromatography.
Results: The positive rates of EV71 IgG, IgM and IgA antibodies were 62.07%(72/116), 68.10%(79/116) and 60.34%(70/116), respectively,and the positive rate of EV71 nucleic acid test was 72.41%(84/116), the difference being not statistically significant(P>0.05).The positive rate of EV71 IgM and IgG antibody in mild children was lower than that in severe children,and the difference was statistically significant(P<0.01).
Conclusion: The diagnostic ability of the detection of EV71 IgG, IgM and IgA antibodies in hand-foot-mouth disease is good; the positive rate of IgM and IgG antibody of severe EV71 is higher than that of common children, which suggests that it has clinical application value for early diagnosis and symptomatic treatment of the disease.

Key words: Hand foot-mouth-disease, EV71；IgG antibody, IgM antibody, IgA antibody, Children