ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2022, Vol. 14 ›› Issue (3): 197-201.doi: 10.3969/j.issn.1674-3865.2022.03.004

• 临床论著 • 上一篇    下一篇

小儿呼吸道合胞病毒肺炎风热郁肺证脂类及氨基酸类代谢组学研究

徐超, 迟磊, 崔振泽   

  1. 116000 辽宁 大连，大连市妇女儿童医疗中心（集团）呼吸科

  • 收稿日期:2021-07-15 出版日期:2022-06-25 上线日期:2023-12-01
  • 通讯作者: 崔振泽，E-mail:cuizhenze@126.com
  • 基金资助:
    国家自然科学基金面上项目（81473726）

Metabonomics research of lipids and amino acids in children with respiratory syncytial virus pneumonia with wind-heat depressing lung syndrome

XU Chao, CHI Lei, CUI Zhenze   

  1. Dalian Women and Children′s Medical Center (Group)，Dalian 116000，China

  • Received:2021-07-15 Published:2022-06-25 Online:2023-12-01
  • Contact: CUI Zhenze，E-mail:cuizhenze@126.com

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摘要： 目的 应用液相色谱-质谱联用(LC-MS)技术研究小儿呼吸道合胞病毒(RSV)肺炎风热郁肺证的脂类及氨基酸类代谢组学特征。方法 选取小儿RSV肺炎风热郁肺证患儿和同期正常儿童各30例，应用LC-MS技术对受试者血清进行检测，筛选RSV肺炎风热郁肺证患儿的脂类和氨基酸类差异性代谢物。结果 两组儿童的血清代谢产物具有明显差异，筛选出主成分的变量重要性值>2的差异性代谢物共5个，其中RSV肺炎风热郁肺证患儿磷脂酰胆碱(PC 34∶3)和苯丙氨酸(Phe-Phe)明显低于正常儿童，鞘磷脂(SM36∶1、SM34∶0)和色氨酸(Tryptophan)明显高于正常儿童，差异均有统计学意义(P<0.05)。结论 基于LC-MS的代谢组学方法可以区分RSV肺炎风热郁肺证和正常儿童，筛选出的差异性代谢物可能是表征小儿RSV肺炎风热郁肺证的潜在诊断标志物。

关键词:

呼吸道合胞病毒肺炎, 风热郁肺证, 代谢组学, 儿童

Abstract:

Objective To study the metabolomic characteristics of lipids and amino acids of children with respiratory syncytial virus(RSV) pneumonia with wind-heat depressing lung syndrome by liquid chromatography-mass spectrometry (LC-MS) technology.Methods A total of 30 children with RSV pneumonia with wind-heat depressing lung syndrome and 30 normal children in the same period were selected.LC-MS technology was used to detect the serum of the subjects,and the differential metabolites in lipids and amino acids in children with RSV pneumonia with wind-heat depressing lung syndrome were screened out.Results The serum metabolites of the two groups of children were significantly different,and a total of 5 differential metabolites with the variable importance value of the principal component >2 were screened out,among which phosphatidylcholine(PC 34∶3) and phenylalanine(Phe-Phe) were significantly lower in RSV children than in normal children,while sphingomyelin(SM36∶1,SM34∶0) and tryptophan(Tryptophan) were significantly higher than in normal children,there being statistically significant differences(P<0.05).Conclusion The metabolomic method based on LC-MS can distinguish children with RSV pneumonia with wind-heat depressing lung syndrome from normal children,and the screened differential metabolites may be potential diagnostic markers for children with RSV pneumonia with wind-heat depressing lung syndrome.

Key words:

Respiratory syncytial virus pneumonia；Wind-heat depressing lung syndrome；Metabonomics；Children

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