ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2022, Vol. 14 ›› Issue (2): 110-114.doi: 10.3969/j.issn.1674-3865.2022.02.004

• 临床论著 • 上一篇    下一篇

miRNA-126及runt相关转录因子3在支气管哮喘患儿外周血中的表达及意义

王珺, 张广超, 陈丹, 孙晓敏   

  1. 450000 郑州，郑州大学附属儿童医院，河南省儿童医院，郑州儿童医院普内一病区

  • 收稿日期:2022-02-21 出版日期:2022-04-25 上线日期:2023-12-01
  • 通讯作者: 孙晓敏，E-mail：Sunney1minmin@163.com
  • 基金资助:
    2019年河南省医学科技攻关计划联合共建项目（LHGJ20190921）

Expression and significance analysis of miRNA-126 and runt-associated transcription factor 3 in peripheral blood of children with bronchial asthma

WANG Jun, ZHANG Guangchao, CHEN Dan, SUN Xiaomin   

  1.  General Internal Medicine, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital,Zhengzhou Children's Hospital,Zhengzhou 450000,China
  • Received:2022-02-21 Published:2022-04-25 Online:2023-12-01
  • Contact: SUN Xiaomin，E-mail：Sunney1minmin@163.com

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摘要： 目的 探讨miRNA-126与runt相关转录因子3(RUNX3)在支气管哮喘患儿中的表达及意义。方法 选取河南省儿童医院收治的80例支气管哮喘患儿为研究对象，根据疾病的临床进展分为急性期组(n=42)与缓解期组(n=38);同期选取健康体检的儿童40例为对照组。qRT-PCR法检测3组受试儿外周血中miRNA-126水平，Western blot检测RUNX3蛋白表达水平；分析哮喘组患儿miRNA-126与RUNX3水平的相关性；ROC曲线分析miRNA-126及RUNX3在哮喘中的诊断价值。结果 与对照组相比，缓解期组与急性期组的miRNA-126表达水平均显著增加(P<0.05),RUNX3蛋白水平显著降低(P<0.05);且急性期组miRNA-126水平显著高于缓解期组，RUNX3水平显著低于缓解期组(P<0.05);miRNA-126与RUNX3水平呈显著负相关(P<0.05);miRNA-126与RUNX3诊断哮喘的AUC分别为0.933,0.847,诊断哮喘临床分期的AUC分别为0.696,0.798。结论 miRNA-126与RUNX3可能协同参与儿童支气管哮喘的发生发展，可作为哮喘诊断及病情评估的潜在辅助指标。 

关键词:

支气管哮喘, miRNA-126, RUNX3, 相关性, 儿童

Abstract:

Objective To investigate the expression and significance of miRNA-126 and RUNX3 in children with bronchial asthma.Methods Eighty children with bronchial asthma treated in Henan Children's Hospital were selected as research subjects,and they were divided into acute stage group(n=42) and remission stage group(n=38) according to the clinical progress of the disease. At the same time, 40 healthy children were selected as the control group. The levels of miRNA-126 in peripheral blood of the three groups were detected by qRT-PCR, and the expression level of RUNX3 protein was detected by Western blot; The correlation between miRNA-126 and RUNX3 level in asthmatic groups was analyzed. The diagnostic value of miRNA-126 and RUNX3 in asthma was analyzed by ROC curve.Results Compared with the control group, the expression level of miRNA-126 was significantly increased(P<0.05), while RUNX3 protein was significantly decreased(P<0.05) in both the remission and acute stage groups. The level of miRNA-126 in acute stage group was significantly higher than that in remission group, while the level of RUNX3 protein was significantly lower than that in remission group (P<0.05).The miRNA-126 was significantly negatively correlated with RUNX3 level (P<0.05). The AUCs of miRNA-126 and RUNX3 in diagnosing asthma were respectively 0.933 and 0.847,and the AUCs for the diagnosis of clinical stages of asthma were respectively 0.696 and 0.798.Conclusion The miRNA-126 and RUNX3 may be synergistically involved in the development of bronchial asthma in children, which may serve as a potential auxiliary indicator for the diagnosis and evaluation of asthma

Key words:

Bronchitical asthma, miRNA-126, RUNX3, Correlation, Children