ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2022, Vol. 14 ›› Issue (2): 110-114.

• 临床论著 • 上一篇    下一篇

miRNA-126及runt相关转录因子3在支气管哮喘患儿外周血中的表达及意义

  

  • 出版日期:2022-04-25 上线日期:2023-12-01

Expression and significance analysis of miRNA-126 and runt-associated transcription factor 3 in peripheral blood of children with bronchial asthma

  • Published:2022-04-25 Online:2023-12-01

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摘要： 目的 探讨miRNA-126与runt相关转录因子3(RUNX3)在支气管哮喘患儿中的表达及意义。方法 选取河南省儿童医院收治的80例支气管哮喘患儿为研究对象，根据疾病的临床进展分为急性期组(n=42)与缓解期组(n=38);同期选取健康体检的儿童40例为对照组。qRT-PCR法检测3组受试儿外周血中miRNA-126水平，Western blot检测RUNX3蛋白表达水平；分析哮喘组患儿miRNA-126与RUNX3水平的相关性；ROC曲线分析miRNA-126及RUNX3在哮喘中的诊断价值。结果 与对照组相比，缓解期组与急性期组的miRNA-126表达水平均显著增加(P<0.05),RUNX3蛋白水平显著降低(P<0.05);且急性期组miRNA-126水平显著高于缓解期组，RUNX3水平显著低于缓解期组(P<0.05);miRNA-126与RUNX3水平呈显著负相关(P<0.05);miRNA-126与RUNX3诊断哮喘的AUC分别为0.933,0.847,诊断哮喘临床分期的AUC分别为0.696,0.798。结论 miRNA-126与RUNX3可能协同参与儿童支气管哮喘的发生发展，可作为哮喘诊断及病情评估的潜在辅助指标。 

关键词:

支气管哮喘, miRNA-126, RUNX3, 相关性, 儿童

Abstract:

ObjectiveTo investigate the expression and significance of miRNA-126 and RUNX3 in children with bronchial asthma.MethodsEighty children with bronchial asthma treated in Henan Children's Hospital were selected as research subjects,and they were divided into acute stage group(n=42) and remission stage group(n=38) according to the clinical progress of the disease. At the same time, 40 healthy children were selected as the control group. The levels of miRNA-126 in peripheral blood of the three groups were detected by qRT-PCR, and the expression level of RUNX3 protein was detected by Western blot; The correlation between miRNA-126 and RUNX3 level in asthmatic groups was analyzed. The diagnostic value of miRNA-126 and RUNX3 in asthma was analyzed by ROC curve.ResultsCompared with the control group, the expression level of miRNA-126 was significantly increased(P<0.05), while RUNX3 protein was significantly decreased(P<0.05) in both the remission and acute stage groups. The level of miRNA-126 in acute stage group was significantly higher than that in remission group, while the level of RUNX3 protein was significantly lower than that in remission group (P<0.05).The miRNA-126 was significantly negatively correlated with RUNX3 level (P<0.05). The AUCs of miRNA-126 and RUNX3 in diagnosing asthma were respectively 0.933 and 0.847,and the AUCs for the diagnosis of clinical stages of asthma were respectively 0.696 and 0.798.ConclusionThe miRNA-126 and RUNX3 may be synergistically involved in the development of bronchial asthma in children, which may serve as a potential auxiliary indicator for the diagnosis and evaluation of asthma

Key words:

Bronchitical asthma, miRNA-126, RUNX3, Correlation, Children