定喘颗粒对呼吸道合胞病毒肺炎幼鼠肺组织病毒载量及气道阻力的影响

摘要/Abstract

摘要： 目的探讨定喘颗粒对呼吸道合胞病毒(RSV)肺炎幼鼠肺组织病毒载量、炎症因子及气道阻力的影响。方法选取30只SD幼龄大鼠随机分为3组，正常组、RSV肺炎组、定喘颗粒干预组，每组10只。采用滴鼻法建立RSV肺炎模型；采用双腔体描记法检测幼龄大鼠气道阻力，苏木精-伊红染色法观察肺组织病理改变；ELISA法检测肺泡灌洗液(BALF)中白细胞介素-8(IL-8)、肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)含量。结果 (1)病毒载量方面：与正常组比较，RSV肺炎组、定喘颗粒干预组病毒载量均增高，差异有统计学意义(P<0.05);与RSV肺炎组比较，定喘颗粒干预组病毒载量明显降低，差异有统计学意义(P<0.05)。(2)气道阻力方面：与正常组比较，RSV肺炎组、定喘颗粒干预组气道阻力均增高，差异有统计学意义(P<0.05);与RSV肺炎组比较，定喘颗粒干预组气道阻力明显降低，差异有统计学意义(P<0.05)。(3)病理损伤及干湿比方面：与正常组比较，RSV肺炎组、定喘颗粒干预组病理损伤评分及肺湿干比均增高，差异有统计学意义(P<0.05);与RSV肺炎组比较，定喘颗粒干预组病理损伤评分及肺湿干比均降低，差异有统计学意义(P<0.05)。(4)BALF中IL-8、TNF-α、IL-1β的含量方面：与正常组比较，RSV肺炎组、定喘颗粒干预组IL-8、TNF-α、IL-1β含量明显升高，差异有统计学意义(P<0.05);与RSV肺炎组比较，定喘颗粒干预组IL-8、TNF-α、IL-1β含量明显降低，差异有统计学意义(P<0.05)。结论定喘颗粒干预后可降低肺组织病毒载量，降低气道阻力，改善肺损伤程度，抑制幼鼠RSV肺炎炎症因子水平。

关键词: 呼吸道合胞病毒, 定喘颗粒, 肺组织炎症因子, 气道阻力, 大鼠

Abstract: ObjectiveTo investigate the effects of Dingchuan granules on viral load, inflammatory factors and airway resistance in lung tissue of young rats with respiratory syncytial virus(RSV) pneumonia.MethodsThirty young SD mice were randomly divided into three groups: normal group, RSV pneumonia group, and Dingchuan granules intervention group, with 10 mice in each group. Establish RSV pneumonia model using nasal drip method. Double chamber tracing method was used to detect airway resistance in mice, and hematoxylin eosin staining (HE staining) method was used to observe pathological changes in lung tissue. ELISA method was used for detecting the content of interleukin-8 (IL-8) and tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) in alveolar lavage fluid(BALF). Results（1）Viral load: Compared with the normal group, RSV pneumonia group and Dingchuan granules intervention group had higher viral load (P<0.05).Compared with the RSV pneumonia group, the viral load in the Dingchuan granules intervention group was significantly lower (P<0.05). （2） In terms of airway resistance: Compared with the normal group, both the RSV pneumonia group and the Dingchuan granules intervention group showed an increase in airway resistance (P<0.05). Compared with the RSV pneumonia group, the airway resistance of the Dingchuan granules intervention group was significantly reduced (P<0.05). （3） In terms of pathological injury and dry-to-wet ratio: Compared with the normal group, the RSV pneumonia group and the Dingchuan granules intervention group showed an increase in pathological injury score and lung wet-to-dry ratio (P<0.05). Compared with the RSV pneumonia group, the pathological injury score and lung wet-dry ratio of the Dingchuan granules intervention group were both reduced (P<0.05). （4）In terms of IL-8 ,TNF-α and IL-1β content in BALF: Compared with the normal group, the RSV pneumonia group and the Dingchuan granules intervention group had significant increase in IL-8，TNF-α and IL-1β content (P<0.05). Compared with the RSV pneumonia group, the Dingchuan granules intervention group showed significant decrease in the content of IL-8, TNF-α and IL-1β(P<0.05). ConclusionDingchuan granules can reduce the viral load in lung tissue, reduce airway resistance, improve the degree of lung injury, and inhibit the level of inflammatory factors of RSV pneumonia in young rats.

Key words:

Respiratory syncytial virus, Dingchuan granules, Inflammatory factors in lung tissue, Airway resistance, Rat

周涛, 张秀英, 王雪峰, 王咏雪, 赵航宇, 魏晨浩, . 定喘颗粒对呼吸道合胞病毒肺炎幼鼠肺组织病毒载量及气道阻力的影响[J]. 中国中西医结合儿科学, 2024, 16(1): 26-30.

ZHOU Tao, ZHANG Xiuying, WANG Xuefeng, WANG Yongxue, ZHAO Hangyu, WEI Chenhao. . Effects of Dingchuan granules on viral load and airway resistance in lung tissue of young rats with respiratory syncytial virus pneumonia[J]. Chinese Pediatrics of Integrated Traditional and Western Medicine, 2024, 16(1): 26-30.

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定喘颗粒对呼吸道合胞病毒肺炎幼鼠肺组织病毒载量及气道阻力的影响

Effects of Dingchuan granules on viral load and airway resistance in lung tissue of young rats with respiratory syncytial virus pneumonia

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