ISSN 1674-3865  CN 21-1569/R
主管：国家卫生健康委员会
主办：中国医师协会
　　　辽宁省基础医学研究所
　　　辽宁中医药大学附属医院
中国中西医结合儿科学

中国中西医结合儿科学 ›› 2024, Vol. 16 ›› Issue (5): 419-424.doi: 10.3969/j.issn.1674-3865.2024.05.013

• 临床研究 • 上一篇    下一篇

中枢性性早熟女童肠道菌群分析

王格，张雪荣，刘雁云，段云雁，沈鹤霄   

  1. 430065 武汉，湖北中医药大学基础医学院2021级中医诊断学专业研究生（王格）；湖北中医药大学，湖北时珍实验室（张雪荣，刘雁云）；430060 武汉，湖北中医药大学附属医院（湖北省中医院）儿科，中医肝肾研究及应用湖北省重点实验室（张雪荣，段云雁）；430206 武汉，美益添生物医药（武汉）有限公司（沈鹤霄）

  • 收稿日期:2024-01-03 修回日期:2024-02-16 出版日期:2024-10-25 上线日期:2024-10-25
  • 通讯作者: 张雪荣，E-mail：1251635860@qq.com
  • 基金资助:
    湖北省中医药管理局2023~2024年度中医药面上项目(ZY2023M065)； 湖北省中医药管理局中医药重点学科建设项目(鄂中医通[2023]2号)； 湖北中医药大学“十四五”优秀学科团队建设项目(100702020803)；

Analysis of intestinal flora of girls with central precocious puberty

WANG Ge1，ZHANG Xuerong1,2,3，LIU Yanyun1,2，DUAN Yunyan3，SHEN Hexiao4   

  1. 1Hubei University of Traditional Chinese Medicine,Wuhan 430065,China; 2Hubei Shizhen Laboratory,Wuhan 430065,China; 3Hubei Provincial Hospital of Traditional Chinese Medicine,Affiliated Hospital of Hubei University of Chinese Medicine,Hubei Key Laboratory of theory and application research of liver and kidney in traditional Chinese medicine，Wuhan 430060,China; 4Meiyitian Biopharmaceutical (Wuhan) Co.,Wuhan 430206,China
  • Received:2024-01-03 Revised:2024-02-16 Published:2024-10-25 Online:2024-10-25
  • Contact: ZHANG Xuerong，E-mail：1251635860@qq.com

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摘要： 目的：分析中枢性性早熟(CPP)女童肠道菌群的多样性、结构及功能特征，挖掘其与同年龄段健康女童的差异菌群及潜在功能。方法：选择2023年11～12月在湖北省中医院儿科门诊就诊并确诊为CPP的女童20例为CPP组，同期选取同年龄段健康女童志愿者20例为对照组。收集两组粪便样本进行16S rRNA测序，分析两组肠道菌群组成结构及功能差异。结果：两组肠道菌群的α多样性比较差异无统计学意义(P>0.05)。CPP组肠道菌群的β多样性显示出明显的分离趋势，两组比较差异有统计学意义(P<0.05)。CPP组的基因富含内分泌和代谢疾病、核苷酸代谢、氨基酸代谢、细胞加工及信号转导等功能。结论：CPP女童与健康女童在肠道菌群数目、种类多样性、分布均匀性方面差异不大，但在结构上存在显著差异；预测内分泌和代谢疾病、核苷酸代谢、氨基酸代谢、细胞加工及信号转导等功能与CPP有潜在相关性。

关键词: 中枢性性早熟, 肠道菌群, 功能预测, 儿童

Abstract: Objective：To analyze the diversity,structure and functional characteristics of the intestinal flora of girls with central precocious puberty (CPP),and to explore the differential flora and potential functions of them with those of healthy girls of the same age.Methods：We selected 20 girls who attended the pediatric outpatient clinic of Hubei Provincial Hospital of Traditional Chinese Medicine from November to December 2023 and were diagnosed with CPP as the CPP group,and 20 healthy girls volunteers of the same age during the same period were included as the control group.Stool samples were collected for 16S rRNA sequencing to analyze the differences in the composition,structure and function of the intestinal flora between the two groups.Results：The difference in α-diversity of intestinal flora between the two groups was not statistically significant (P>0.05).The β-diversity of intestinal flora in the CPP group showed a clear tendency to segregate,and the difference between the two groups was statistically significant (P<0.05).Genes in the CPP group were enriched for endocrine and metabolic disorders,nucleotide metabolism,amino acid metabolism,cellular processing,and signal transduction.Conclusion：Differences between girls with CPP and healthy girls are not significant in terms of the number,species diversity,and distributional homogeneity of intestinal flora,but there are significant structural differences;the functions in predicting endocrine and metabolic diseases,nucleotide metabolism,amino acid metabolism,cellular processing,and signaling are potentially correlated with CPP.

Key words: Central precocious puberty, Intestinal flora, Function prediction