ISSN 1674-3865  CN 21-1569/R
主管:国家卫生和计划生育委员会
主办:中国医师协会
   辽宁省基础医学研究所
   辽宁中医药大学附属医院

Chinese Pediatrics of Integrated Traditional and Western Medicine ›› 2021, Vol. 13 ›› Issue (1): 1-.doi: 10.3969/j.issn.1674-3865.2021.01.001

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Role of miRNAs in the model of asthma in mice

ZHANG Dongjiao,XU Hao   

  1. Pediatric Department,Central Hospital Affiliated to Shenyang Medical Colloge,Shenyang 110024,China
  • Received:2020-09-08 Published:2021-02-25 Online:2021-05-17
  • Contact: XU Hao,E-mail:xuhao6668@126.com

Abstract: Objective:To investigate the differential expression of miRNAs in asthma model of mice.Methods:BALB/c mice were randomly divided into normal control group, asthma group(ovalbumin treatment group) and treatment group(dexamethasone treatment group), 8 mice in each group. Eosinophil(EOS), neutrophil(NEU), macrophage(MAC) and lymphocyte(LYM) in BALF of each group were detected by hematology analyzer, and the expression of IL-4,IL-6 and IL-13 in BALF of each group were detected by ELISA. miRNAs microarray was used to analyze the differential expression of miRNAs in different groups of lung tissues; the correlation between miR-455 and EOS,NEU,MAC,LYM,IL-4,IL-6 and IL-13 was statistically analyzed.Results:The expression of EOS,NEU,MAC,LYM,IL-4,IL-6 and IL-13  in the asthma group was significantly higher than that in the control group, and the expression of various factors was downregulated in treatment group compared with asthma group. The expression of multiple miRNAs in asthma group changed,and miR-455 was up-regulated significantly in asthma group. miR-455 was positively correlated with the expression of EOS,NEU,MAC,LYM,IL-4,IL-6 and IL-13.Conclusion:miR-455 is significantly up-regulated in ovalbumin-induced asthma model of mice.

Key words: Asthma, MicroRNA, Inflammatory factor, Cytokine