Abstract: Objective：To analyze the diversity,structure and functional characteristics of the intestinal flora of girls with central precocious puberty (CPP),and to explore the differential flora and potential functions of them with those of healthy girls of the same age.Methods：We selected 20 girls who attended the pediatric outpatient clinic of Hubei Provincial Hospital of Traditional Chinese Medicine from November to December 2023 and were diagnosed with CPP as the CPP group,and 20 healthy girls volunteers of the same age during the same period were included as the control group.Stool samples were collected for 16S rRNA sequencing to analyze the differences in the composition,structure and function of the intestinal flora between the two groups.Results：The difference in α-diversity of intestinal flora between the two groups was not statistically significant (P>0.05).The β-diversity of intestinal flora in the CPP group showed a clear tendency to segregate,and the difference between the two groups was statistically significant (P<0.05).Genes in the CPP group were enriched for endocrine and metabolic disorders,nucleotide metabolism,amino acid metabolism,cellular processing,and signal transduction.Conclusion：Differences between girls with CPP and healthy girls are not significant in terms of the number,species diversity,and distributional homogeneity of intestinal flora,but there are significant structural differences;the functions in predicting endocrine and metabolic diseases,nucleotide metabolism,amino acid metabolism,cellular processing,and signaling are potentially correlated with CPP.

Key words: Central precocious puberty, Intestinal flora, Function prediction